essential [Pubmed|12682299]

resistance to cerulenin [Pubmed|11325930][Pubmed|24641521]

essential [Pubmed|12682299]

CRISPRi knock-down: terminal phenotype of growth cessation, moderate bending [Pubmed|27238023]

FabF-I109F: resistance to cerulenin [Pubmed|11325930,24641521]

FabF-L112F, FabF-I109M: resistance to cerulenin (Zeigler DR, unpublished observations)

11325930, 18384517, 12837788, 12737802, 12682299, 21383089, 24641521, 21542858, 22178969, 24641521

11325930, 18384517, 12837788, 12737802, 12682299, 21383089, 24641521, 21542858, 22178969, 24641521, 27238023

BEC11340 (lacA::Pxyl-dcas9 amyE::Pveg-sgRNA(fabF) trpC2) [http://www.bgsc.org/getdetail.php?bgscid=BEC11340 BGSC], [Pubmed|27238023]

1A573 (fabF2 trpC2) [http://www.bgsc.org/getdetail.php?bgscid=1A573 BGSC], cerulenin-resistant mutant

1A576 (fabF14 trpC2) [http://www.bgsc.org/getdetail.php?bgscid=1A576 BGSC], cerulenin-resistant mutant

1A577 (fabF20 trpC2) [http://www.bgsc.org/getdetail.php?bgscid=1A577 BGSC], cerulenin-resistant mutant

pGP3509 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP3510: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [https://www.thermofisher.com/order/catalog/product/K30001#/K30001 pETSUMOadapt], available in [SW|Jörg Stülke]'s lab

pGP3511: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab